DNase I

Genetic polymorphism of human serum DNase I in Chinese populations

中国人血清脱氧核糖核酸酶I（DNaseI）的遗传多态性研究

Optimizing DNase I Dosage in Non-kit Labeling System of Genomic Probe

基因组探针非试剂盒标记系统中DNaseI用量优化

RNA Responsible for Conferring a DNase I Sensitive Structure on Albumin Gene in Assembled Chromatin

RNA促进小鼠重组染色质白蛋白基因DNaseⅠ消化敏感性

Comparative Study on Template Activity and Susceptibility to DNase I Digestion of Mouse Liver and Ascites Hepatoma Cell Chromatin

小鼠腹水肝癌细胞染色质的转录活性及对DNaseⅠ消化敏感性的研究

Analyses of Three-stranded DNA with DNase I and Fluorescence

三链DNA的酶解荧光检测

We have modified the procedure to a sequential digestion of papain and DNase I for taking into account the fast traditional trypsin digestion .

考虑到传统的胰酶消化速度不好控制，我们用木瓜酶结合DNA酶I序贯消化。

The enzyme vigour definition of DNAse I by Kunitz and Yasuda etc. can be as an active detection system of new DNA enzyme ;

利用Kunitz、Yasuda等对DNAseⅠ的酶活力定义作为新型DNA酶样物质的活性检测体系；

Methods The preparing parameters were optimised by assays of DNA precipitation , DNA gel retardation , DNase I protection , and electron transmission microscope .

方法以DNA沉淀试验、DNA阻滞试验、DNaseⅠ保护试验和电镜分析对该疫苗颗粒的制备参数进行优化。

The condition of peptide-DNA vaccine preparation was determined by precipitation assay , gel retardation assay , DNase I protection assay and electronic microscopy .

DNA沉淀分析、结合多肽增量分析、DNA凝胶阻滞、DNaseⅠ保护试验及电镜分析优化疫苗颗粒制备条件；

The β - globin gene LCR consists of four erythroid-specific DNase I hypersensitive sites ( HS1-HS4 ), every HS activity is defined to its core sequences .

LCR由4个DNase历高敏位点（5'HS1-5'HS4）构成，每个HS的活性又都集中在核心序列上。

The results demonstrate that DNA methylase activity of rat liver of Diabetic Nephropathy is obviously lower than the control , and its genome DNA possesses higher sensitivity to Dnase I than that of the control .

结果显示：糖尿病肾病大鼠肝DNA甲基化酶活性明显低于正常鼠肝，其基因组DNA对DNase敏感性比正常大鼠肝增强；

THe effects of KSYS compound on transcription activities , digestive sensitivity of chromatin DNase I and chromatin chemical components in liver nuclei both of young and old rats were studied in this experiment .

本文研究抗衰延寿方对青、老年大鼠肝细胞核转录活性、染色质DNaseI消化敏感性及染色质化学组分的影响。

The mitochondrial DNA ( mtDNA ) from liver of Silurus asotus had been isolated and purified by the method of density gradient centrifugation and DNase I , RNase digestion .

采用差速离心法及DNaseⅠ、RNase消化法制备并纯化了鲇（SilurusAsotus）肝脏线粒体DNA（mitochondrialDNA，mtDNA）。

The results showed that the digestive sensitivity of chromatin DNase I and the content of non-histone chromatin protein in liver nuclei of young rats were obviously higher than those of old ones ( p < 0.01 ) .

结果证明：青年大鼠肝细胞核染色质DNaseI消化敏感性和肝细胞核非组蛋白染色体蛋白含量均明显的高于老年大鼠（P<0.01）。

The PBMCs were isolated from these patients ' blood samples separately and the total RNA was extracted from PBMCs . DNase I without RNase was used to eliminate the remained DNA genome and then reverse transcript cDNA .

分别提取其PBMCs中的总RNA，经无RNA酶的DNA酶Ⅰ消化去除残存的基因组DNA后逆转录合成cDNA。

After incubation with DNase I for 30 minutes , naked DNA was completely degraded while DNA in complexes MLV - DNA ( 3:1 ) and SLV - DNA ( 3:1 ) was almost intact .

与DNase温育30min后，裸DNA（C组）被完全降解，而MLV-DNA（3∶1）和SLV-DNA（3∶1）中DNA基本保持完整。

The transcription activities and the digestive sensitivity of chromatin DNase I in liver nuclei of both young and old rats fed with KSYS compound were enhanced obviously , and the content of non-histone chromatin protein was not changed .

服用抗衰延寿方后，青、老年大鼠肝细胞核转录活性、肝细胞核染色质DNasI消化敏感性均明显提高，而非组蛋白染色体蛋白含量无改变。

Dead spermatozoa did not complete the internalization process . The highest positive rate ( before DNase I digestion ) was found in membrane-broken spermatozoa as a result of freeze-thawing and this was independent of the sperm donors .

死精子不能完成外源DNA的内化过程，但反复冻融导致质膜破裂的死精子具有更高的结合率，而且阳性率与动物个体无关。

Kinetic study on limited DNase I digestion revealed that the chromatin from hepatoma cells was more susceptible to DNase I di - gestion , indicating a structural difference between the transcription - ally active regions of both chromatins .

肝细胞染色质对DNaseⅠ消化更加敏感，说明肝癌细胞染色质转录活性部分的结构与正常肝染色质不同。